Quantifying MAMP-induced Production of Reactive Oxygen Speci

发布日期：2025-11-04 17:33 来源：bio-protocol 作者：Rozalynne Samira 浏览次数：

Quantifying MAMP-induced Production of Reactive Oxygen Species in Sorghum and Maize

高粱和玉米中由MAMP诱导产生的活性氧定量分析

Plants have the ability to recognize microbe-associated molecular patterns (MAMPs) and mount a defense response. The level of the MAMP response can vary depending on genetic and environmental factors. The most commonly studied MAMPs are flg22, a peptide epitope from bacterial flagellin, and chitin, a component of the fungal cell wall. Protocols for measuring reactive oxygen species (ROS) production elicited by flg22 and chitin in maize and sorghum are described.植物能够识别微生物相关分子模式（MAMPs）并启动防御反应。MAMP反应的水平可因遗传和环境因素而异。研究最多的MAMPs是细菌鞭毛蛋白肽段flg22和真菌细胞壁成分几丁质。本文描述了在玉米和高粱中通过flg22和几丁质诱导活性氧（ROS）产生的检测方法。

Samira, R., Zhang, X., Kimball, J., Cui, Y., Stacey, G. and Balint-Kurti, P. J. (2019). Quantifying MAMP-induced Production of Reactive Oxygen Species in Sorghum and Maize. Bio-101: e3304. DOI: 10.21769/BioProtoc.3304.

方法概要：