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(ISP)国际链霉菌计划培养基

发布日期:2020-07-24 09:19 来源:International Journal of Syste 作者:Shirling E B 浏览次数:
Shirling E B, Gottlieb D  Methods for characterization of Streptomyces species[J]  International Journal of Systematic Bacteriology, 1966, 16(3): 313-340

Trace salts solution(用于ISP3ISP4ISP5ISP7
FeSO4·7H2O  0.1 g
MnCl2·4H2O   0.1 g
ZnSO4·7H2O  0.1 g
Distilled water 100.0 mL

ISP 1: Tryptone-yeast extract broth
Bacto-Tryptone         5.0 g
Bacto-Yeast Extract     3.0 g
Distilled water          1.0 liter
pH 7 0-7 2 before autoclaving

ISP 2: Yeast extract-malt agar
Bacto-Yeast Extract (Difco)    4.0 g
Bacto-Malt Extract (Difco)    10.0 g
Bacto-Dextrose (Difco)          4.0 g
Distilled water                        1.0 liter
Adjust to pH 7 3, then add
Bacto agar                           20.0 g
Liquefy by steaming at 100°C for 15-20 minutes.

ISP 3: Oatmeal agar
Oatmeal              20.0 g
Agar                   18.0 g
Cook or steam 20 g oatmeal in 1000 ml distilled water for 20 minutes
Filter through cheese cloth
Add distilled water to restore volume of filtrate to 1000 mL
Trace salts solution     1.0 mL
Adjust to pH 7 2 with NaOH
Add 18 g agar; liquefy by steaming at 100°C for 15-20 minutes.

ISP 4: Inorganic salts-starch agar
Solution Ⅰ: Difco soluble starch     10.0 g
Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 mL.
Solution Ⅱ:
K2HPO4 (anhydrous basis)             1.0 g
MgSO4·7H2O                                  1.0 g
NaCl                                                 1.0 g
(NH4)2SO4                                      2.0 g
CaCO3                                             2.0 g
Distilled water                                  500 mL
Trace salts solution                           1.0 ml
pH should be between 7 0 and 7 4. Do not adjust if it is within this range.
Mix starch suspension and salts solution.
Add agar                                         20.0 g
Liquefy agar by steaming at 100°C for 15-20 minutes.

ISP 5: Glycerol-asparagine agar
L-asparagine (anhydrous basis)     1.0 g
Glycerol                                        10.0 g
K2HPO4 (anhydrous basis)           1.0 g
Distilled water                              1.0 liter
Trace salts solution                      1.0 mL
The pH of this solution is about 7.0-7.4. Do not adjust if it is within this range.
Agar                                             20.0 g
Liquefy agar by steaming at 100°C for 15-20 minutes.

ISP 6: Peptone-yeast extract iron agar
Bacto-Peptone Iron Agar, dehydrated (Difco)    36.0 g
Bacto-Yeast Extract (Difco)                    1.0 g
Distilled water                                         1.0 liter
pH should be 7 0-7 2 before autoclaving; adjust if necessary.
Liquefy agar by steaming at 100°C for 15-20 minutes.

ISP 7: Tyrosine agar
Glycerol                             15.0 g
L-tyrosine (Difco)                0.5 g
L-asparagine (Difco)           1.0 g
K2HPO4 (anhydrous basis)      0.5 g
MgSO4·7H2O                   0.5 g
NaCl                                  0.5 g
FeSO4·7H2O                  0.01 g
Distilled water                   1.0 liter
Trace salts solution           1.0 mL
Adjust to pH 7 2-7 4
Bacto-Agar                        20 0 g
Liquefy by steaming at 100°C for 15-20 minutes.
 
Standard 90 mm dishes containing 25 ml per dish will be needed for each culture.
Sterilization should be performed under high pressure at 121oC for 20 minutes, unless otherwise stated.

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