P-Iodonitrotetrazolium Violet

发布日期：2013-05-17 10:32 来源：研究小组作者：admin 浏览次数：

CAS Number: 146-68-9

Synonyms: INT, 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride

Product Description

Appearance: Light yellow powder

Molecular formula: C19H13C1IN5O2

Molecular weight: 505.7

Melting point: (with decomp.) 202-204°C

E1%(249nm) = 680 (water)

Storage and Stability It is stable at least two years if stored at 2-8°C in the dark.

Solubility/Solution Stability

INT dissolves in water to give a clear light yellow to light green solution at 4 mg/mL, although gentle heat and sonication are necessary for this concentration. INT dissolves up to 8 mg/mL in ethanol, and up to 30 mg/mL in ethylene glycol monomethyl ether. Aqueous solutions are reported to be stable for several weeks when stored at 2-8°C in the dark.

General Remarks

This tetrazolium dye precursor forms a purple formazan dye on reduction and has been used in a variety of applications. It is considered to have higher reactivity than some tetrazolium compounds, at least with respect to succinate dehydrogenase, with optimal results obtained using a concentration of 0.8 mM INT. The formazan (available as I7375) is comparatively insoluble in water, so can be detected visually or extracted into ethanol or organic solvent and determined spectrophotometrically. Altman reviewed properties of a number of different tetrazolium salts.

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