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Fusarium fruiting body microbiome member Pantoea agglomerans

发布日期:2022-06-10 15:44 浏览次数:

Fusarium fruiting body microbiome member Pantoea agglomerans inhibits fungal pathogenesis by targeting lipid rafts

Abstract

Plant-pathogenic fungi form intimate interactions with their associated bacterial microbiota during their entire life cycle. However, little is known about the structure, functions and interaction mechanisms of bacterial communities associated with fungal fruiting bodies (perithecia). Here we examined the bacterial microbiome of perithecia formed by Fusarium graminearum, the major pathogenic fungus causing Fusarium head blight in cereals. A total of 111 shared bacterial taxa were identified in the microbiome of 65 perithecium samples collected from 13 geographic locations. Within a representative culture collection, 113 isolates exhibited antagonistic activity against F. graminearum, with Pantoea agglomerans ZJU23 being the most efficient in reducing fungal growth and infectivity. Herbicolin A was identified as the key antifungal compound secreted by ZJU23. Genetic and chemical approaches led to the discovery of its biosynthetic gene cluster. Herbicolin A showed potent in vitro and in planta efficacy towards various fungal pathogens and fungicide-resistant isolates, and exerted a fungus-specific mode of action by directly binding and disrupting ergosterol-containing lipid rafts. Furthermore, herbicolin A exhibited substantially higher activity (between 5- and 141-fold higher) against the human opportunistic fungal pathogens Aspergillus fumigatus and Candida albicans in comparison with the clinically used fungicides amphotericin B and fluconazole. Its mode of action, which is distinct from that of other antifungal drugs, and its efficacy make herbicolin A a promising antifungal drug to combat devastating fungal pathogens, both in agricultural and clinical settings.

植物病原真菌在其整个生命周期中与其相关的细菌微生物群形成密切的相互作用。然而,关于与真菌子实体(perithecia)相关的细菌群落的结构、功能和相互作用机制知之甚少。在这里,我们检查了由禾谷镰刀菌形成的外皮细菌微生物群,禾谷镰刀菌是导致谷物镰刀菌青枯病的主要病原真菌。在从13个地理位置收集的65个样本的微生物组中,共鉴定出111个共享细菌类群。在一个代表性的培养物中,113个分离株对禾谷镰刀菌具有拮抗活性,其中成团泛菌 ZJU23 在减少真菌生长和感染性方面最有效。 Herbicolin A被鉴定为ZJU23分泌的关键抗真菌化合物。遗传和化学方法导致了其生物合成基因簇的发现。 Herbicolin A 在体外和植物体内对各种真菌病原体和抗杀菌剂分离物显示出有效的功效,并通过直接结合和破坏含有麦角甾醇的脂筏发挥真菌特异性作用模式。此外,与临床使用的杀菌剂两性霉素 B 和氟康唑相比,herbicolin A对人类机会性真菌病原体烟曲霉和白色念珠菌表现出显着更高的活性(高5至141倍)。其作用方式与其他抗真菌药物不同,其功效使herbicolin A成为一种有前途的抗真菌药物,可在农业和临床环境中对抗破坏性真菌病原体。


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Fusarium fruiting body microbiome member Pantoea agglomerans inhibits fungal pathogenesis by targeting lipid rafts Nature Microbiology

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