Assessment of Antifungal Efficacy and Release Behavior of Fu

发布日期：2024-06-13 19:54 来源：网络作者：Kumar, Ravinder 浏览次数：

Assessment of Antifungal Efficacy and Release Behavior of Fungicide-Loaded Chitosan-Carrageenan Nanoparticles against Phytopathogenic Fungi
Polymers 2022, 14(1), 41; https://doi.org/10.3390/polym14010041

Biopolymeric Chitosan-Carrageenan nanocomposites 66.6-231.82 nm in size containing the chemical fungicide mancozeb (nano CSCRG-M) were synthesized following a green chemistry approach. The physicochemical study of nanoparticles (NPs) was accomplished using a particle size analyzer, SEM and FTIR. TEM exhibited clover leaf-shaped nanoparticles (248.23 nm) with mancozeb on the inside and entrapped outside. Differential scanning calorimetry and TGA thermogravimetry exhibited the thermal behaviour of the nanoform. Nano CSCRG-1.5 at 1.5 ppm exhibited 83.1% inhibition against Alternaria solani in an in vitro study and performed as well as mancozeb (84.6%). Complete inhibition was exhibited in Sclerotinia sclerotiorum at 1.0 and 1.5 ppm with the nanoformulation. The in vivo disease control efficacy of mancozeb-loaded nanoparticles against A. solani in pathogenized plants was found to be relatively higher (79.4 +/- 1.7) than that of commercial fungicide (76 +/- 1.1%) in pot conditions. Nanomancozeb showed superior efficacy for plant growth parameters, such as germination percentage, root-shoot ratio and dry biomass. The nanoformulation showed higher cell viability compared to mancozeb in Vero cell cultures at 0.25 and 0.50 mg/mL in the resazurin assay. CSCRG-0.5 showed slow-release behavior up to 10 h. Thus, these green nano-based approaches may help combat soil and water pollution caused by harmful chemical pesticides.
采用绿色化学方法合成了粒径为66.6 ~ 231.82 nm的壳聚糖-卡拉胶生物高分子复合材料(纳米CSCRG-M)。利用粒径分析仪、扫描电镜(SEM)和红外光谱(FTIR)对纳米颗粒(NPs)进行了理化研究。透射电镜显示了四叶草形状的纳米颗粒(248.23 nm)，内层包裹着代森锰锌，外层包裹着代森锰锌。差示扫描量热法和热重热分析显示了纳米形态的热行为。在体外研究中，纳米CSCRG-1.5在1.5 ppm时对茄疫病菌的抑制作用为83.1%，对代森锰锌的抑制作用为84.6%。纳米配方在1.0和1.5 ppm时对菌核菌有完全抑制作用。在盆栽条件下，载锰锌纳米颗粒对病原菌的体内防效(79.4 +/- 1.7)高于市售杀菌剂(76 +/- 1.1%)。对发芽率、根冠比、干生物量等植物生长指标均有显著影响。在reazurin实验中，纳米制剂在0.25和0.50 mg/mL的Vero细胞培养中显示出比代森锰锌更高的细胞活力。CSCRG-0.5表现出长达10 h的缓释行为。因此，这些基于绿色纳米的方法可能有助于对抗有害化学农药造成的土壤和水污染。